UNDERGROUND GARDEN CLUB

WELCOME TO THE ⚘ UNDERGROUND GARDEN CLUB ⚘

Let's Get Growing

Microbe-based Proteins

Alternatives to Plant & Animal Proteins

Gyser Microbes that Make Edible Protein

Fungi Flour for Protein Rich Bread

Plant Anatomy Engineering

Drought Resistance to Capturing Carbon

Helping Plants Survive Droughts

Ft. Prof. Jenn Brophy

Enhanced CO2 Capturing Trees

Green House Gas Removal

CO2, Methane (CH4), and NO2

CO2 Mineralizing Bacteria in Crop Fields

Atmospheric Methane Eating Bacteria

FYI LOTS OF WORK STILL TO BE DONE HERE

Frontier Materials

Rethinking Fashion and Function

Bio-Luminescent Embelshiments

Ft. Aradhita Parasrampuria

Affordable Caskets using Fungi/Mycellium

Environmental Stewardship & Healing

Observing and Rejuvinating

Soil, Air, and Water Stewardship

Ft. Soverign Science

Permaculture and Health

Ft. Down the Carrot Hole Documentary

Starting Your Journey

Introductory Learning and Setup

Join the Learning Club

GPT4 Science Tutor + Siri

Audio-Based AI Assistance

Getting Up to Speed on Bio

Lectures 1-3 @ MIT Opencourseware

Introductory Bio/Chem

Lectures 1-3 @ MIT Opencourseware

Start with a Community Lab

Intro Education/Connections

Intro Lab Learning/Bootcamps

@ Your Nearest Community Bio-Lab

Genspace (NYC) →Biotech Without Borders (NYC) →Counter Culture Labs (Oakland) →

Search for community bio labs near you!

...Or Start Your Own Lab

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Prepping a WorkTable

Using Epoxy to Encase a Surface

Airflow and Filteration

Crash Course in Contamination

Minimal Reagents and Equipments

Just enough for Intro Experiments

Accessing Biologicals & Chemicals

How to Buy from New England Bioscience and Addgene

Introductory Experiments

Building Intuition with Biological and Physical Worlds

Join the Learning Club

Biological Machines

Intro Experiments Pt.1

Biological Materials

Intro Experiments Pt.2

3D Printing Crash Course

Setup and Overview of How to Print

Bio-Plastic Membership Card

(Deli Potatoes + Homemade Glycerol)

Fundamentals of Materials Structure

Unit 1 @ MIT Opencourseware

Transform Bacteria to Glow Green

Inserting GFP DNA

Using Bacteria as Bio-Factories

Replicating & Harvesting GFP DNA

Tools for Transformation

Intro Experiments Pt.3

Build a Still Air Box Workspace

Cheap Semi-Sterilized Workspace

Build Smartphone Florescence Microscope

Intro to Quantum Mechanics + Electrical Work

Deep Dive Experiments

Dig Deeper and Create New Paths

Contribute Quests & Learning

Plant Structures

@ NY Botanical Gardens (Online)

Soil Science 1: Physical Properties

@ NY Botanical Gardens (Online)

Botanical Pharmacy

ft. Open Plant @ Genspace

Growing Marchantia Made Easy

(From Thallus Cliipings or Gemmae)

Backing Up Plant Species

AKA Storing Plants in a Freezer

Genetically Editing Marchantia to be Red

Pt.1 of Gene Editing w/ Agrobacterium

Designing DNA Parts for Gene Editing Plasmid

Chosing a plasmid, genes, and signaling DNA strands

Building the Gene Editing Plasmid from DNA Fragments

Processes for Combinging DNA Fragments (PCR, Restriction Digest)

GMO'ing Marchantia: via Spores

Agrobacteria horizontal gene transfer in plant spores

GMO'ing Marchantia: via Thallus

Agrobacteria horizontal gene transfer in plant clippings

Home Grown Medicine with Marchantia

Pt.2 of Gene Editing w/ Agrobacterium

Home Grown Insulin

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Home Grown Testosterone

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Home Grown Estrogen

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Plant Anatomy Engineering

GMO'ing Massive Mutant Plants

Speed Breeding Cabinet

(Ft. John Innes Institute)

Repressive Gene Knockout

(w/ CRISPR)

Polyploiding

Food Justice

Food Nutrition Densitiy

Measuring with Refactometers

Environmental Justice

Environmental Data Stewardship

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Sampling

Soil Sampling

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Water Sampling

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Air Sampling

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Environmental Defense and Case Building

How to Leverage Federal Laws/Regulations

Microbe-Brewery Crew

Cellular Fermentation of Chemicals & Food

Starter Bio-Reactor = Pio-Reactors

Don't build your own bio-reactor (yet)

Brewing a Simple Protein

Automating Yeast Growth + GFP Florescence

Bio-based Haber-Bosch

Eznymes Produced w/ Bacteria

Bacteria-based Jet Fuel

Produced w/ Bacteria. Seriously.

Spider Silk via Yeast

ft. Open Plant @ Genspace

Edible Protein Production

Proteins Produced w/ Yeast

Massive Edible Protein Production

Turning 55 Gallon Oil Drums into Bio-Reactors

Bio-Prospecting v. Bio-Piracy

Who Owns the Rights to Mexico’s ‘Wonder’ Plant?

Sequestration

Atmoshperic CO2, CH4, N2O Removal